Sysmex Inostics - Innovative technologies for better diagnosis

BEAMing Technology

BEAMing allows the quantitative detection of cancer gene mutations with high sensitivity and selectivtiy.

The workflow of BEAMing includes the following steps :

  • Pre-Amplification
  • Emulsion PCR
  • Hybridization
  • Flow Cytometry

 

Quick Facts

BEAMing stands for Beads, Emulsions, Amplification, and Magnetics. For more information see Dressman D. et al. (2003)

  • It is a technology to perform single-molecule PCRs on magnetic beads in water-in-oil emulsions.
  • The core process is the transformation of a population of DNA molecules into a population of beads each coated with thousands of copies of identical sequence.
  • The technology has the capacity to detect and enumerate mutant and wild-type DNA when present at ratios greater than 1:10,000.
  • BEAMing has unparalled sensitivity and selectivity (100-fold higher than conventional technologies).

BEAMing Principle Hybridization Detail_multiplex_generic_small

 

For more information please download our fact sheet BEAMing Digital PCR_Technology  [pdf].